Remember, bacteria can't start to colonize without a food source...so adding things like biospira or the like should only be done just before you add fish, or if you plan to add ammonia yourself.
The bacteria, also, cannot live long without a food source...so the ammonia source must remain constant.
I can't answer your ? about how much (PURE) ammonia to add, but with your test kit (which you really should have if you don't already) get the ammonia betwee 3-5ppm and keep it there. Depending how much bacteria you have beginning to colonize will determine how ofter you need to keep adding ammonia. For the first while you won't have to add much (or any), but then as things get started you'll have to add more and more.
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