To me it sounds like you are done as long as everything stays at zero. I put the pleco in mine a few days before I added a few more fish and then I put a filter from my turtle tank onto my 55 gallon and the very next day everything was at zero and has stayed that way for a while.
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55 Gallon
...1 Pleco
...4 Sunshine Peacocks
...1 Altolamprologus Calvus
...1 Cyrtocara Moorii (Blue Dolphin)
...1 Julidochromis Marlieri Burundi
...1 Neolamprologus brichardi
...1 Yellow Lab
...1 Sciaenochromis fryeri (Chewere)
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