If you decide to use the bio spira, you will only need the 1 oz. size which is the smallest size. It treats 30 gallons. Make sure the dealer you get it from is reputable and has handled the product properly. The dealers receive it from Marineland refrigerated, and it should be kept that way until it is used. When they receive their supply, it should be immediately refrigerated by the dealer, and when you buy it, you should immediately take it home and put it in the fridge. If you won't be going home right away, or it's a lengthy ride home, put it in a cooler with a little bit of ice. If it's not handled properly, you will pay for a package of dead bacteria that won't cycle your tank. Also, the product is meant to be added to your tank with a full bio load of fish, so when you decide on what all your fish are gonna be for that tank, add all of these fish and the bio spira at the same time. If you want to take an extra precaution to make sure the bio spira is alive, you can add pure clear ammonia to the tank until your ammonia reading is 5 ppm, after adding the bio spira (don't add the fish if you are doing this), and if the ammonia reading is at zero in 24 hours, you know it's alive and working, and you can add your fish.
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